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Molecular Techniques

DNA extraction from peripheral blood using RELIAPREP

  • from a maximum of 3ml of blood obtaining approximately 100ug of DNA
  • ­extraction with ReliaPrep ™ Large Volume HT gDNA Isolation System, based on magnetic beads extraction
  • ­Automated extraction

DNA extraction from peripheral blood using MAXWELL

  • ­ from a maximum of 0.4ml of blood obtaining approximately 11ug DNA
  • ­ extraction with Maxwell ® 16 Blood DNA Purification Kit, based on magnetic beads extraction
  • Semi-automated extraction

DNA Quality Control (electrophoresis, Dropsense, Picogreen)

  • Electrophoresis in 0.8% agarose gel, 13 samples.
  • Determination of the nucleic acid concentration by spectrophotometer using DropSense96 (concentration, purity: ratio A260/280 and A230/260, analysis of contaminants)
  • Determination of the dsDNA concentration with Quant-iT™ PicoGreen® dsDNA Assay Kit.

Isolation of serum and plasma

  • PLP / Plasma low platelet content from 8ml blood in a PST tube -> approximately 4 aliquots of 0.5 ml, double centrifugation to obtained a content of platelets <10,000/μL.
  • PHP/ Plasma high platelet content from 8ml blood in a PST tube -> approximately 4 aliquots of 0.5 ml, single centrifugation.
  • Serum from 8.5 ml of blood in a SST tube -> approximately 4 aliquots of 0.5 ml.

Price

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Generalitat de Catalunya

Unió Europea

CSIC

Universitat Autònoma de Barcelona

Ajuntament de Badalona

Institut Català de la Salut

Germans Trias i Pujol Hospital

Fundació Institut d'investigació en Ciències de la Salut Germans Trias i Pujol