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Cellular Techniques

Isolation and cryopreservation of peripheral blood mononuclear cells (PBMCs -BUFFY COAT)

  • From 10 ml of blood in a EDTA tube
  • ­ 2 aliquots of 0,5 ml in cryopreserved medium.
  • ­ The storage fee is charged separately.

Blood cryopreservation

  • From 6ml of blood in an ACD tube
  • ­ Cryopreservation 1:1 in RPMI + DMSO 20%
  • ­ 2 aliquots of 1 ml
  • ­ The storage fee in Nitrogen liquid is charged separately.

Immortalization of B cells
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  • From 10 ml of fresh blood
  • ­ Isolation of PBMCs and transformation of B cells with EBV B95.8 virus.

Immortalization of B cells (technique in validation phase)
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  • From 1 ml cryopreserved blood
  • ­ Positive selection of B cells after transformation with EBV B95.8 virus.

Price

See price list


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Generalitat de Catalunya

Unió Europea

CSIC

Universitat Autònoma de Barcelona

Ajuntament de Badalona

Institut Català de la Salut

Germans Trias i Pujol Hospital

Fundació Institut d'investigació en Ciències de la Salut Germans Trias i Pujol